apc anti mouse cd80  (Elabscience Biotechnology)

Journal: Cells

Article Title: Cancer Immunomodulatory Effect of Bidens pilosa L. in Mice: Suppression of Tumor-Associated Macrophages and Regulatory T Cells

doi: 10.3390/cells15020126

Figure Lengend Snippet: Expression levels of mRNAs and proteins associated with TAMs and Tregs in BPA -treated mouse tumors. ( A ) Quantification of mRNA by qRT-PCR for TAM/Treg-associated tumor-promoting factors (Arg-1, YM-1, CCL2, CCL22, Foxp3, CD25, TGF-β, CXCR3, CCR4, CCR8, CCR10) and tumor-inhibitory factors (TNF-α, IL-1β, iNOS, CXCL10, CCR7). Data were normalized to 18 S rRNA and are shown relative to the model group (baseline control). ( B ) Representative immunoblots of tumor tissue for TAM- and Treg-associated proteins. Pro-tumor: CD206, Foxp3, CD25, PD-1, PD-L1; Anti-tumor: CD80, CD4, GITR. ( C ) Quantification of protein expression from Western blots. Values were normalized to α-tubulin, β-actin, or GAPDH as indicated. Statistical significance is denoted as follows ( n = 6): compared to the model group, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: To block Fc receptors, cells were incubated with anti-CD16/32 (Elabscience, Cat. E-AB-F0997A, Wuhan, China) for 10 min. After washing, cells were stained with PerCP/Cyanine 5.5 anti-mouse F4/80 (Elabscience, Cat. E-AB-F0995J), FITC anti-mouse CD206 antibody (Elabscience, Cat. E-AB-F1135C), and APC anti-mouse CD80 antibody (Elabscience, Cat. E-AB-F0992E) at 4 °C for 30 min.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot

Journal: Materials Today Bio

Article Title: Biomimetic nanovesicle vaccines derived from dendritic cells sensitized with whole tumor antigens for melanoma immunotherapy

doi: 10.1016/j.mtbio.2025.102409

Figure Lengend Snippet: Evaluation of the effect of nanovaccines on activating immunity. (A) Representative scatter plots and proportions of BMDCs mature cells after nanovaccine stimulation; (B) CLSM images showing the uptake ability of nanovaccines by DCs. Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (blue), lysosomes with Lyso Tracker Green (green), and nanovaccines with DiI (red) (scale bar: 20 μm); (C) The proportion of CD11c + CD86 + CD80 + cells in BMDCs detected by FCM (n = 3). (D) Representative scatter plots of differentiation effects of splenic lymphocytes analyzed by FCM; (E) The frequencies of CD3 + CD8 + cells in splenic lymphocytes. The data were analyzed using an ordinary one-way ANOVA comparison test. Data are presented as mean ± SD. Differences with P < 0.05 (∗), P < 0.01 (∗∗), P < 0.001 (∗∗∗), P < 0.0001 (∗∗∗∗) were regarded as statistically significant, and ns means no significance.

Article Snippet: Horseradish peroxidase (HRP)-conjugated secondary antibodies and APC anti-mouse CD80 were purchased from Proteintech (USA).

Techniques: Labeling, Comparison

Journal: Materials Today Bio

Article Title: Biomimetic nanovesicle vaccines derived from dendritic cells sensitized with whole tumor antigens for melanoma immunotherapy

doi: 10.1016/j.mtbio.2025.102409

Figure Lengend Snippet: Nanovaccines exhibit trained immunity. (A) Overview of the in vitro training model; (B) Representative scatter plots and frequencies of CD11c + CD80 + CD86 + cells in BMDCs after LPS restimulation (n = 3); (C) FCM analysis displaying frequencies of CD11c + CD80 + CD86 + cells in BMDCs; (D) Overview of the training in vivo model; (E) Expression levels of cytokines in serum after LPS restimulation (n = 3); (F) Adaptive immune response in vivo training model description and the test results of IFN-γ expression in splenic lymphocytes. The data were analyzed using an ordinary one-way ANOVA comparison test. Data are presented as mean ± SD. Differences with P < 0.05 (∗), P < 0.01 (∗∗), P < 0.001 (∗∗∗), P < 0.0001 (∗∗∗∗) were regarded as statistically significant.

Article Snippet: Horseradish peroxidase (HRP)-conjugated secondary antibodies and APC anti-mouse CD80 were purchased from Proteintech (USA).

Techniques: In Vitro, In Vivo, Expressing, Comparison

Journal: Materials Today Bio

Article Title: Biomimetic nanovesicle vaccines derived from dendritic cells sensitized with whole tumor antigens for melanoma immunotherapy

doi: 10.1016/j.mtbio.2025.102409

Figure Lengend Snippet: The efficacy of immune training is associated with the STING pathway. (A) WB assay for protein expression in BMDCs after LPS restimulation; (B–E) Expression levels of cytokines in serum after LPS restimulation (n = 3); FCM analysis displaying frequencies of MHC II + (F), CD40 + (G), CD80 + (H) and CD86 + (I) cells in BMDCs. The data were analyzed using an ordinary one-way ANOVA comparison test. Data are presented as mean ± SD. Differences with P < 0.05 (∗), P < 0.01 (∗∗), P < 0.001 (∗∗∗), P < 0.0001 (∗∗∗∗) were regarded as statistically significant.

Article Snippet: Horseradish peroxidase (HRP)-conjugated secondary antibodies and APC anti-mouse CD80 were purchased from Proteintech (USA).

Techniques: Expressing, Comparison